Difference between revisions of "Part:BBa K2660009"
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| − | This part can be used with nMag (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2660008">BBa_K2660008</a></html>) to join different domains of a protein of interest when irradiated by blue light | + | This part was engineered to be a building block of the standard BioBrick framework, and can be used with nMag (<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2660008">BBa_K2660008</a></html>) to join different domains of a protein of interest when irradiated by blue light |
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Revision as of 13:21, 14 October 2018
pMag
- Light-activated protein
This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools.The positive Magnet (pMag) when irradiated by a blue light change through electrostatic interactions.
This part was engineered to be a building block of the standard BioBrick framework, and can be used with nMag (BBa_K2660008) to join different domains of a protein of interest when irradiated by blue light
References
Kawano, F. et al. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat. Commun. 6:6256 doi: 10.1038/ncomms7256 (2015)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 87
- 1000COMPATIBLE WITH RFC[1000]