Difference between revisions of "Part:BBa K2841514"
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<partinfo>BBa_K2841514 short</partinfo> | <partinfo>BBa_K2841514 short</partinfo> | ||
| − | A gRNA which targets the LacIq promoter for the LacI protein (specifically designed for the Z1 cassette). When the gRNA is combined with a dCAS9 (<bbpart>BBa_K2841510</bbpart>) the LacI protein will be repressed, which means every Lac promoter will be induced. (Targets a LacIq promoter (<bbpart>BBa_K2841517</bbpart>) with the sequence: CGTTGACACCATCGAATG<b>GTGCAAAACCTTTCGCGGTA</b>TGGCATGATAGCGCCCGGAAGA) | + | A gRNA which targets the LacIq promoter (<bbpart>BBa_K2841517</bbpart>) for the LacI protein (specifically designed for the Z1 cassette). When the gRNA is combined with a dCAS9 (<bbpart>BBa_K2841510</bbpart>) the LacI protein will be repressed, which means every Lac promoter will be induced. (Targets a LacIq promoter (<bbpart>BBa_K2841517</bbpart>) with the sequence: CGTTGACACCATCGAATG<b>GTGCAAAACCTTTCGCGGTA</b>TGGCATGATAGCGCCCGGAAGA) |
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Latest revision as of 20:53, 13 October 2018
LacI Repressing gRNA
A gRNA which targets the LacIq promoter (BBa_K2841517) for the LacI protein (specifically designed for the Z1 cassette). When the gRNA is combined with a dCAS9 (BBa_K2841510) the LacI protein will be repressed, which means every Lac promoter will be induced. (Targets a LacIq promoter (BBa_K2841517) with the sequence: CGTTGACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGA)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 102
Illegal NgoMIV site found at 131 - 1000COMPATIBLE WITH RFC[1000]