Difference between revisions of "Part:BBa K2841513"
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A fusion of <bbpart>BBa_K2841512</bbpart> and <bbpart>BBa_K2841511</bbpart>. A self-terminating self-cleaving gRNA cis acting factor (a guide RNA without the targeting region.) | A fusion of <bbpart>BBa_K2841512</bbpart> and <bbpart>BBa_K2841511</bbpart>. A self-terminating self-cleaving gRNA cis acting factor (a guide RNA without the targeting region.) | ||
| − | Simply put the complementary sequence of the DNA that you would like the gRNA to target just before this biobrick part, and express <bbpart>BBa_K2841510</bbpart>. The dCAS9 will localise to your target. | + | Simply put the complementary sequence of the DNA that you would like the gRNA to target just before this biobrick part, and express <bbpart>BBa_K2841510</bbpart>. The dCAS9 will localise to your target. You do not need a terminator - this part will self terminate! |
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Revision as of 19:06, 13 October 2018
Self-Cleaving gRNA Kit
A fusion of BBa_K2841512 and BBa_K2841511. A self-terminating self-cleaving gRNA cis acting factor (a guide RNA without the targeting region.)
Simply put the complementary sequence of the DNA that you would like the gRNA to target just before this biobrick part, and express BBa_K2841510. The dCAS9 will localise to your target. You do not need a terminator - this part will self terminate!
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 82
Illegal NgoMIV site found at 111 - 1000COMPATIBLE WITH RFC[1000]