Difference between revisions of "Part:BBa K2841510"
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<partinfo>BBa_K2841510 short</partinfo> | <partinfo>BBa_K2841510 short</partinfo> | ||
| − | The dCAS9 protein used in the [https://www.ncbi.nlm.nih.gov/pubmed/23761437 CRISPR repressor 2013 paper]. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (<bbpart>BBa_K2841514</bbpart>) which directs it to a site of interest. The [https://www.rcsb.org/structure/4ZT9 structure for the protein] family has been solved. | + | The dCAS9 protein used in the[https://www.ncbi.nlm.nih.gov/pubmed/23761437 CRISPR repressor 2013 paper]. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (<bbpart>BBa_K2841514</bbpart>) which directs it to a site of interest. The [https://www.rcsb.org/structure/4ZT9 structure for the protein] family has been solved. |
https://static.igem.org/mediawiki/parts/6/66/T--Warwick--2018-4zt9.gif | https://static.igem.org/mediawiki/parts/6/66/T--Warwick--2018-4zt9.gif | ||
Revision as of 18:46, 13 October 2018
Streptococcus Pyogenes dCAS9
The dCAS9 protein used in theCRISPR repressor 2013 paper. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (BBa_K2841514) which directs it to a site of interest. The structure for the protein family has been solved.
The protein is shown in wheat colour BBa_K2841510. The RNA cis acting factor (for the dCAS9 protein to recognise the gRNA) is in grey BBa_K2841514. The gRNA's targeting region (what specifies the target sequence) is in green BBa_K2841514.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]