Difference between revisions of "Part:BBa K2841510"

Revision as of 18:46, 13 October 2018

Streptococcus Pyogenes dCAS9

The dCAS9 protein used in the CRISPR repressor 2013 paper. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (BBa_K2841514) which directs it to a site of interest. The structure for the protein family has been solved.

The protein is shown in wheat colour BBa_K2841510. The RNA cis acting factor (for the dCAS9 protein to recognise the gRNA) is in grey BBa_K2841514. The gRNA's targeting region (what specifies the target sequence) is in green BBa_K2841514.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 18:46, 13 October 2018 (view source) Psychosahedron (Talk \| contribs) ← Older edit		Revision as of 18:46, 13 October 2018 (view source) Psychosahedron (Talk \| contribs) Newer edit →
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	https://static.igem.org/mediawiki/parts/6/66/T--Warwick--2018-4zt9.gif		https://static.igem.org/mediawiki/parts/6/66/T--Warwick--2018-4zt9.gif
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	The protein is shown in wheat colour <bbpart>BBa_K2841510</bbpart>. The RNA cis acting factor (for the dCAS9 protein to recognise the gRNA) is in grey <bbpart>BBa_K2841514</bbpart>. The gRNA's targeting region (what specifies the target sequence) is in green <bbpart>BBa_K2841514</bbpart>.		The protein is shown in wheat colour <bbpart>BBa_K2841510</bbpart>. The RNA cis acting factor (for the dCAS9 protein to recognise the gRNA) is in grey <bbpart>BBa_K2841514</bbpart>. The gRNA's targeting region (what specifies the target sequence) is in green <bbpart>BBa_K2841514</bbpart>.