Difference between revisions of "Part:BBa K2549039"
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<partinfo>BBa_K2549039 short</partinfo> | <partinfo>BBa_K2549039 short</partinfo> | ||
| − | This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64 ([[Part:BBa_K2549057]]), dNLS ([[Part:BBa_K2549056]]) and ZF21.16 ([[Part:BBa_K2549046]]), from N terminal to C terminal. VP64 is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp, it is cleaved and the transcription activation function cannot be conducted. | + | This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64 ([[Part:BBa_K2549057]]), dNLS ([[Part:BBa_K2549056]]) and ZF21.16 ([[Part:BBa_K2549046]]), from N terminal to C terminal. VP64 is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp ([[Part:BBa_K2549041]]), it is cleaved and the transcription activation function cannot be conducted. |
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===Biology=== | ===Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:28, 11 October 2018
VP64-dNLS-ZF21.16
This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64 (Part:BBa_K2549057), dNLS (Part:BBa_K2549056) and ZF21.16 (Part:BBa_K2549046), from N terminal to C terminal. VP64 is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp (Part:BBa_K2549041), it is cleaved and the transcription activation function cannot be conducted.
Biology
==
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 502
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]