Coding
ZF21.16

Part:BBa_K2549046

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-07)


ZF21.16

This part is a synthetic engineered zinc finger which can bind to ZF21.16 binding sites with high specificity. It can also function with other synthetic zinc fingers with high orthogonality, thus enabling us to construct our zinc finger-based combiner. It can also be utilized by other iGEM teams to build their own zinc finger-based genetic circuits in mammalian cells. We include this part as it is required for the parts collection Part:BBa_K2549016 ~ Part:BBa_K2549043. It was developed for the project Swords http://2017.igem.org/Team:Fudan .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None