Difference between revisions of "Part:BBa K2810002"
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The RTBV promotor is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence. | The RTBV promotor is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence. | ||
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This promoter is supposed to cause gene expression only in phloem tissue, as previously documented [https://www.ncbi.nlm.nih.gov/pubmed/8220476 here.] Its original host is the Rice Tungro Bacilliform Virus. | This promoter is supposed to cause gene expression only in phloem tissue, as previously documented [https://www.ncbi.nlm.nih.gov/pubmed/8220476 here.] Its original host is the Rice Tungro Bacilliform Virus. | ||
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Revision as of 10:47, 10 October 2018
RTBV Vascular Specific Promotor
The Rice Tungro Bacilliform Virus (RTBV) promotor has a vascular expression pattern in plants. This promotor has been added to the registry to facilitate expression of genes into plant vascular tissues.
The RTBV promotor is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence.
Usage and Biology
This promoter is supposed to cause gene expression only in phloem tissue, as previously documented here. Its original host is the Rice Tungro Bacilliform Virus. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 611
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 495
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]