Difference between revisions of "Part:BBa J45001"

Revision as of 21:37, 9 March 2008

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

BBa_J45001 encodes SAM salicylic acid carboxyl methyltransferase I derived from SAMT from Antirrhinus majus (snapdragon). SAMT catalyzes the conversion of salicylic acid to methyl salicylate. Methyl salicylate has a wintergreen smell.

Usage and Biology

The culture medium of E. coli cells expressing SAMT contained methyl salicylate (2.1 μg/ml) when the growing medium was supplemented with 5 μg/ml salicylic acid, and methyl benzoate (0.86 μg/ml) when the growing medium was supplemented with 5 μg/ml benzoic acid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 577
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 901
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 471
Illegal SapI.rc site found at 75

Functional Parameters

ec_num	none
kegg	none
protein	SAMT
swisspro	Q8H6N2

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-#The culture medium of ''E. coli'' cells expressing ''SAMT'' contained methyl salicylate (2.1 &mu;g/ml) when the growing medium was supplemented with 5 &mu;g/ml salicylic acid, and methyl benzoate (0.86 &mu;g/ml) when the growing medium was supplemented with 5 &mu;g/ml benzoic acid.
+The culture medium of ''E. coli'' cells expressing ''SAMT'' contained methyl salicylate (2.1 &mu;g/ml) when the growing medium was supplemented with 5 &mu;g/ml salicylic acid, and methyl benzoate (0.86 &mu;g/ml) when the growing medium was supplemented with 5 &mu;g/ml benzoic acid.
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