Difference between revisions of "Part:BBa J45999:Experience"

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===Applications of BBa_J45999===
 
===Applications of BBa_J45999===
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Odor-free chassis for odor biosynthetic devices and systems.
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[[Image:BBa J45999GC.png|thumb|600px|left|Gas chromatography data of "wildtype" ''E. coli'' and an indole knockout strain of '''Escherichia coli'' (BBa_J45999).]]
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<partinfo>BBa_J45999 AddReview 5</partinfo><br>
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<I>[[User:Rshetty|Reshma Shetty]]</I>
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The odor-free chassis (<partinfo>BBa_J45999</partinfo>) has no smell and produces no detectable levels of indole.
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==Characterization==
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===Gas chromatography analysis of odor-free chassis===
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{{:IGEM:MIT/2006/Odor-free chassis}}
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Revision as of 20:09, 9 March 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J45999

Odor-free chassis for odor biosynthetic devices and systems.


User Reviews

UNIQ492204dd4856f304-partinfo-00000000-QINU

•••••

Reshma Shetty

The odor-free chassis (BBa_J45999) has no smell and produces no detectable levels of indole.

UNIQ492204dd4856f304-partinfo-00000003-QINU

Characterization

Gas chromatography analysis of odor-free chassis

Odor-free chassis does not produce the fecal odor indole
To verify our selection of E. coli strain YYC912 as an odor-free chassis, we analyzed cultures of each strain for indole production using gas chromatography. E. coli strain YYC912 had no detectable indole (A) whereas E. coli strain TOP10 had high levels of indole (B). The retention time of the indole peak from E. coli strain TOP10 is identical to that of the pure indole standard (C).