Difference between revisions of "Part:BBa J45992"
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<partinfo>BBa_J45992 short</partinfo>
 
<partinfo>BBa_J45992 short</partinfo>
  
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This promoter is active in stationary phase and under high osmotic pressure conditions. See [[Part:BBa_J45992:Experience|experience section]] for device characterization using <bbpart>J45995</bbpart> (promoter attached to GFP).
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<partinfo>BBa_J45992</partinfo> is a stationary phase promoter derived from the promoter that controls transcription of ''osmY'' in ''Escherichia coli'' <cite>Yim-1992, Yim-1994<cite>.  <partinfo>BBa_J45992</partinfo> is active in stationary phase and under high osmotic pressure conditions.
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===Usage and Biology===
 
===Usage and Biology===
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This promoter is significantly more active in sationary phase.  Schellhorn, et. al were able to connect the promoter to the LacZ gene in strains grown in rich media. They were able to show that the beta-Galactosidase activity in stationary phase was 408.8 Miller Units, while the activity was only 8.6 Miller Units in exponential phase.  This preferential activation of the osmY promoter is the result of a complex network of inhibitors and activators which act on the promoter during various stages of the cell cycle.  The promoter is specifically inhibited by H-NS, Lrp, cAMP-CRP, and IHF, while it is specifically activated by the sigma s factor, which is strongly activated by rpoS in stationary phase or under starvation conditions (Neidhart 1502-1504).
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Expression of ''osmY'' is dependent on &sigma;<sup>S</sup> ''in vivo'' <cite>Hengge-Aronis-1993</cite>.  Schellhorn ''et al.'' previously demonstrated that an ''osmY::lacZ'' fusion generated the highest transcriptional signal in stationary phase as compared to nine other &sigma;<sup>S</sup>-dependent promoter-''lacZ'' fusions <cite>Schellhorn-1998, Vijayakumar-2004</cite>.  In addition, the ''osmY::lacZ'' fusion generated only a small transcriptional signal during exponential growth.
  
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<biblio>
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#Yim-1994 pmid=8282684
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#Yim-1992 pmid=1317380
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#Hengge-Aronis-1993 pmid=8416901
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#Vijaykumar-2004 pmid=15576800
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#Schellhorn-1998 pmid=9829938
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</biblio>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 23:26, 7 March 2008

Full-length stationary phase osmY promoter

BBa_J45992 is a stationary phase promoter derived from the promoter that controls transcription of osmY in Escherichia coli Yim-1992, Yim-1994<cite>. BBa_J45992 is active in stationary phase and under high osmotic pressure conditions.

Usage and Biology

Expression of osmY is dependent on σS in vivo <cite>Hengge-Aronis-1993. Schellhorn et al. previously demonstrated that an osmY::lacZ fusion generated the highest transcriptional signal in stationary phase as compared to nine other σS-dependent promoter-lacZ fusions Schellhorn-1998, Vijayakumar-2004. In addition, the osmY::lacZ fusion generated only a small transcriptional signal during exponential growth.

<biblio>

  1. Yim-1994 pmid=8282684
  2. Yim-1992 pmid=1317380
  3. Hengge-Aronis-1993 pmid=8416901
  4. Vijaykumar-2004 pmid=15576800
  5. Schellhorn-1998 pmid=9829938

</biblio>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]