Difference between revisions of "Part:BBa J45017:Design"
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===Design Notes===
 
===Design Notes===
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PchA (isochorismate synthase) catalyzes the conversion of chorismate to isochorismate.  PchB (isochorismate-pyruvate lyase) catalyzes the conversion of isochorismate to salicylate.
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*Constructed by PCR
  
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Co-expression of the two proteins from one BioBrick part successfully generates salicylate in ''E. coli''.  For additional design features see [http://parts.mit.edu/wiki/index.php/MIT_2006 the MIT iGEM 2006 wiki].
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====Template====
 +
*pME3366 plasmid
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**We thank Dr. Cornelia Reimmann, Department of Fundamental Microbiology, Universite de Lausanne for kindly providing the pME3366 plasmid encoding the ''pchBA'' genes.  Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].
 +
 
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====Forward primer====
 +
 
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====Reverse primer====
  
 
===Source===
 
===Source===
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PchB and PchA are found in ''Pseudomonas aeruginosa''.  The pME3366 plasmid from which <partinfo>BBa_J45017</partinfo> was constructed was kindly sent to the MIT 2006 iGEM team by Dr. Cornelia Reimmann, Department of Fundamental Microbiology, Universite de Lausanne.  Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].
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''pchBA'' genes from ''Pseudomonas aeruginosa''.   
  
 
===References===
 
===References===

Revision as of 22:11, 7 March 2008

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 1596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Constructed by PCR

Template

  • pME3366 plasmid
    • We thank Dr. Cornelia Reimmann, Department of Fundamental Microbiology, Universite de Lausanne for kindly providing the pME3366 plasmid encoding the pchBA genes. Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].

Forward primer

Reverse primer

Source

pchBA genes from Pseudomonas aeruginosa.

References

Original paper describing pchBA Serino-MolGenGenet-1995. Information about the enzyme encoded by PchA Gaille-JBC-2003.

<biblio>

  1. Serino-MolGenGenet-1995 pmid=7500944
  2. Serino-JBacteriol-1997 pmid=8982005
  3. Gaille-JBC-2002 pmid=11937513
  4. Gaille-JBC-2003 pmid=12624097
  5. Zaitseva-JBC-2006 pmid=16914555

</biblio>