Difference between revisions of "Part:BBa K2549014"

Revision as of 04:46, 7 October 2018

TEV protease N-terminal fragment

This part is N-terminal fragment of TEVp (Part:BBa_K2549013). TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. In out project, TEVpN was fused to CfaN (Part:BBa_K2549009) intein, when co-expressed with CfaC (Part:BBa_K2549010) + TEVpC (Part:BBa_K2549015) in the same cell, will reconstitute site-specific protease activity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 316

Usage and Biology

TBA

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2549014 short</partinfo>
-This part is N-terminal fragment of TEVp([[Part:BBa_K2549013|Click here to see!]]). TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. TEVp'N can be fused to Cfa'N([[Part:BBa_K2549009|Click here to see!]]) intein.
+This part is N-terminal fragment of TEVp ([[Part:BBa_K2549013]]). TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. In out project, TEVpN was fused to CfaN ([[Part:BBa_K2549009]]) intein, when co-expressed with CfaC ([[Part:BBa_K2549010]]) + TEVpC ([[Part:BBa_K2549015]]) in the same cell, will reconstitute site-specific protease activity.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2549014 SequenceAndFeatures</partinfo>
+<!-- Add more about the biology of this part here -->
+===Usage and Biology===
+TBA
@@ Line 16: / Line 16: @@
 ===Functional Parameters===
 <partinfo>BBa_K2549014 parameters</partinfo>
-<!-- -->
+ -->
+===References===