Difference between revisions of "Part:BBa J45014:Design"

Revision as of 21:20, 7 March 2008

alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 99
Illegal BamHI site found at 1426
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534

BBa_J45014 differs from BBa_J45006 by a single point mutation at base pair 1148 (T -> C).

5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA GAA AAA TC -3'

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ggg cct aaa agg aga gct ttg -3'

ATF1 gene in Saccharomyces cerevisiae.

</biblio>

@@ Line 6: / Line 6: @@
 ===Design Notes===
-*This part differs from ATF1 [https://parts.igem.org/wiki/index.php/Part:BBa_J45006 BBa_J45006] at one base pair, (1148; T -> C).
+*<partinfo>BBa_J45014</partinfo> differs from <partinfo>BBa_J45006</partinfo> by a single point mutation at base pair 1148 (T -> C).
-*It is currently in the '''pSB1A3-1''' BioBrick backbone.
-*new forward primer: 5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA GAA AAA TC</b> -3'
+====New forward primer====
-*new reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT <b>TAa ggg cct aaa agg aga gct ttg</b> -3'
+<code>
+' - GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GAA TGA AAT CGA TGA GAA AAA TC''' -3'
+</code>
+====New reverse primer====
+<code>
+'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT '''TAa ggg cct aaa agg aga gct ttg''' -3'
+</code>
 ===Source===
-This part comes from <partinfo>BBa_J45006</partinfo>.
+''ATF1'' gene in ''Saccharomyces cerevisiae''.
 ===References===