Difference between revisions of "Part:BBa J45993:Design"

Revision as of 19:57, 4 February 2008

Minimal stationary phase osmY promoter

Assembly Compatibility:

5'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3'

5'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3'

This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
+==Forward Primer==
+'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3'
-Total Length: 49 bp
+==Reverse Primer==
+'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3'
-Annealing Length: 21 bp
-GC Content: 38.1%
-Melting Temperature: 49.5 degrees C
-hairpin deltaG: -2.66 kcal/mol
-self dimer deltaG: -99.97 kcal/mol
-Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
-Total Length: 54 bp
-Annealing Length: 25 bp
-GC Content: 20.0%
-Melting Temperature: 46.4 degrees C
-hairpin deltaG: -2.86 kcal/mol
-self dimer deltaG: -99.44 kcal/mol
-heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
 ===Source===