Difference between revisions of "Part:BBa J45992:Design"

(→‎References)
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====Forward Primer====
 
====Forward Primer====
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*5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'
+
*5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'
−
*Total Length: 47 bp
+
−
*Annealing Length: 19 bp
+
−
*GC Content: 52.6%
+
−
*Melting Temperature: 53.4 degrees C
+
−
*hairpin deltaG: -3.99 kcal/mol
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−
*self dimer deltaG: -98.3 kcal/mol
+
  
 
====Reverse Primer====
 
====Reverse Primer====

Revision as of 19:53, 4 February 2008


Full-length stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers used to PCR this part out of the E.coli genome:

Forward Primer

  • 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'

Reverse Primer

  • 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
  • Total Length: 54 bp
  • Annealing Length: 25 bp
  • GC Content: 20.0%
  • Melting Temperature: 46.4 degrees C
  • hairpin deltaG: -2.86 kcal/mol
  • self dimer deltaG: -99.44 kcal/mol
  • heterodimer deltaG with Forward Primer: -99.44 kcal/mol

Source

This part was PCRed out of E. coli's genome with the primers listed in the design notes section.

References

<biblio>

  1. Yim pmid=8282684
  2. Schellhorn pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.