Difference between revisions of "Part:BBa J45002:Design"
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===Source=== | ===Source=== | ||
+ | Species: ''Antirrhinum majus'' (Snapdragon flower) | ||
+ | |||
Genbank accession number(s): [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=9789276 AF198492] | Genbank accession number(s): [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=9789276 AF198492] | ||
− | We got this cDNA from | + | We got this cDNA from Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University. |
===References=== | ===References=== |
Revision as of 20:49, 2 February 2008
SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 970
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 654
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:
<biblio>
- Dudareva pmid=11051108
</biblio>
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA AGT GAT GAA GAA AC
- Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAT CTC CTA CTT AGA GAA ACT AC
- Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
- A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
- The original stop codon (TGA at bp 1093) was deleted and a double TAA stop was inserted
Source
Species: Antirrhinum majus (Snapdragon flower)
Genbank accession number(s): [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=9789276 AF198492]
We got this cDNA from Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University.
References
<biblio>
- Murfitt pmid=11051108
- Dudareva pmid=10852939
</biblio>