Difference between revisions of "Part:BBa J45999:Design"
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===Design Notes===
 
===Design Notes===
−
 
 
* CGSC#: 7602
 
* CGSC#: 7602
 
* Strain Designation
 
* Strain Designation
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**Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028   
 
**Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028   
 
* ID#: 64826  
 
* ID#: 64826  
−
 
−
 
  
 
===Source===
 
===Source===
−
 
+
This strain was obtained from the E.coli Genetic Resources: CGSC, The Coli Genetic Stock Center http://cgsc.biology.yale.edu/.  Strain name is YYC912. [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 CGSC# 7602]
−
This strain was obtained from the E.coli Genetic Resources: CGSC, The Coli Genetic Stock Center http://cgsc.biology.yale.edu/.  Strain YYC912.
+
  
 
===References===
 
===References===
−
 
+
<biblio>
−
Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028
+
#Chang-MolMicrobiol-1994 pmid=8022274
 +
</biblio>

Revision as of 22:12, 1 February 2008

E. coli strain YYC912; Odor-free chassis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • CGSC#: 7602
  • Strain Designation
    • Designation: YYC912
    • Source Person: Cronan & Chang
    • Choice: 1
  • Sex(Hfr,F+,F-,or F'): F-
  • Mutations
    • DE(argF-lac)169
    • LAM-
    • poxB15::lacZ::CmR
    • IN(rrnD-rrnE)1
    • rph-1
    • tnaA5
  • No. of Muts Carried: 6
  • Special Uses: lac fusion-stat.phase promoter
  • Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
  • Isogenic_Partner_Is: ZK126
  • References
    • Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028
  • ID#: 64826

Source

This strain was obtained from the E.coli Genetic Resources: CGSC, The Coli Genetic Stock Center http://cgsc.biology.yale.edu/. Strain name is YYC912. [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 CGSC# 7602]

References

<biblio>

  1. Chang-MolMicrobiol-1994 pmid=8022274

</biblio>