Difference between revisions of "Part:BBa K2810001"
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The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system] is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry. | The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system] is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry. | ||
| − | The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500]) using BsmBI/Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | + | The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 BBa_P10500]) using BsmBI/Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. |
This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page]. | This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page]. | ||
Revision as of 22:10, 4 October 2018
Status: 500
Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
The GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry.
The GUS gene is cloned into the golden gate version of pSB1C3 (BBa_P10500) using BsmBI/Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page].
Using this part, the Cardiff iGEM team of 2018 characterised 35S CaMV promoter, the RTBV promoter, Nopaline synthase terminator, 35S CaMV terminator, and G7 terminator.
Sequence and Features Status: 500 Content-type: text/html
Software error:
Not enough arguments for Range::range at /websites/parts.igem.org/cgi/lib/Range.pm line 250, near "range;" Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.