Difference between revisions of "Part:BBa J45014:Design"

(→‎Source)
(→‎References)
Line 15: Line 15:
  
 
===References===
 
===References===
 +
The publication below reports the use of ATF1 in ''E. coli'' to produce isoamyl acetate from isoamyl alcohol.  Note that the publication date was after the 2006 MIT iGEM designed, constructed and tested <partinfo>BBa_J45014</partinfo>.
 +
<biblio>
 +
#Singh--2007 pmid=17891501
 +
</biblio>

Revision as of 18:20, 26 January 2008


alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

  • This part differs from ATF1 BBa_J45006 at one base pair, (1148; T -> C).
  • It is currently in the pSB1A3-1 BioBrick backbone.

Source

This part comes from BBa_J45006.

References

The publication below reports the use of ATF1 in E. coli to produce isoamyl acetate from isoamyl alcohol. Note that the publication date was after the 2006 MIT iGEM designed, constructed and tested BBa_J45014. <biblio>

  1. Singh--2007 pmid=17891501

</biblio>