Difference between revisions of "Help:Plasmid backbones"
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[[image:PlasmidDNA.png]]
 
[[image:PlasmidDNA.png]]
 
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell] independently of the chromosomal DNA. They are often acquired by bacteria and can bestow the benefit of antibiotic resistance or production of novel proteins.  Most BioBrick parts in the Registry are maintained and propagated on plasmids
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell] independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells.  Most BioBrick parts in the Registry are maintained and propagated on plasmids.  Construction of BioBrick parts, devices and systems usually requires working with plasmids.
  
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*[http://en.wikipedia.org/wiki/Transformation_(genetics) '''Transformation'''] - The process researchers use to incorporate plasmids into cells.
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*'''[[Help:Plasmids/Glossary|Glossary]]''' - What are plasmids, vectors and backbones?
  
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*'''[[Help:Plasmids/Construction Plasmids| Types of plasmids]]''' - What's a construction plasmid? Does "plasmid" mean vector backbone only?
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*'''[[Help:Plasmids/Features|Plasmid features]]''' - Common features of plasmids
  
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*'''[[Help:Plasmids/Features|Plasmid features]]''' - Describes the common features of plasmid
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*'''[[Help:Plasmids/Nomenclature|Plasmid naming]]''' - How BioBrick vectors are named
  
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*'''[[Help:Plasmids/Construction|Constructing new plasmids]]''' - How to construct new BioBrick standard vectors.
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*'''[[Help:Plasmids/Choosing the right plasmid|Choosing the right plasmid]]''' - Choosing which plasmid to use
  
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*'''[[Help:Plasmids/Nomenclature|Plasmid naming]]''' - How BioBrick vectors are named
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*'''[[Help:Plasmids/Finding plasmids|Finding plasmids]]''' - How to locate plasmids in the Registry
  
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*[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid '''Browse plasmid parts'''][[Image:Part icon plasmid.png]]
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*'''[[Help:Plasmids/Construction|Constructing new plasmids]]''' - How to construct new BioBrick plasmids
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*[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid '''Browse plasmid parts'''] [[Image:Part icon plasmid.png]]
  
 
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===Antibiotic Resistance===
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'''All Registry plasmids are available through the use of [[Part:BBa_P1010|BBa_P1010]], [[Part:BBa_I52001|BBa_I52001]] and [[Part:BBa_I52002|BBa_I52002]].  (Information on how to use these parts is available on the relevant part page).'''
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By incorporating an antibiotic-resistance gene on a plasmid, you can select for engineered cells using the appropriate antibiotic. The most commonly used antibiotics are ampicillin ("Amp"), kanamycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Cm").  For more information on which plasmids carry these resistance genes, click the [[Help:Plasmids/Nomenclature |nomenclature]] documentation section.   
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'''All of our plasmids are available through the use of [[Part:BBa_P1010|BBa_P1010]], [[Part:BBa_I52001|BBa_I52001]] and [[Part:BBa_I52002|BBa_I52002]].  (Information on how to use these parts is available on the relevant part page).'''
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Revision as of 05:47, 25 January 2008

PlasmidDNA.png

Plasmids are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Construction of BioBrick parts, devices and systems usually requires working with plasmids.

  • Glossary - What are plasmids, vectors and backbones?

All Registry plasmids are available through the use of BBa_P1010, BBa_I52001 and BBa_I52002. (Information on how to use these parts is available on the relevant part page).