Difference between revisions of "Part:pSB3T5:Experience"
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<partinfo>pSB3T5</partinfo><br>
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<partinfo>pSB3T5 AddReview 4</partinfo><br>
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<I>Reshma Shetty</I>
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<I>[[User:Rshetty|Reshma Shetty]]</I>
 
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When miniprepped, pSB3T5-I52001 does not give as high of yields as expected for a high copy plasmid likely due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin.  I am working to address this issue.  However, this plasmid should still work fine (it is just not as convenient to prep).
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[[Part:pSB3T5|pSB3T5]] is a functional low to medium copy plasmid but does not propagate at high copy.  [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] has been used to successfully assemble BioBrick parts.  However, [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] does not give high miniprep yields due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin.  [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] works fine as a plasmid but is not as convenient for plasmid DNA purification.
 
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Revision as of 01:02, 25 January 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB3T5

User Reviews

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••••

Reshma Shetty

pSB3T5 is a functional low to medium copy plasmid but does not propagate at high copy. pSB3T5-I52001 has been used to successfully assemble BioBrick parts. However, pSB3T5-I52001 does not give high miniprep yields due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin. pSB3T5-I52001 works fine as a plasmid but is not as convenient for plasmid DNA purification.

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