Difference between revisions of "Part:BBa K2835000:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 +
 +
This part contains improved version of the <partinfo>BBa_E1010</partinfo> red flourescent protein.
 
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
 
To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.
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 +
Part is originally derived from GenBank: AF506027.1
  
 
===Source===
 
===Source===
  
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Discosoma striata (coral)
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GenBank: AF506027.1
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https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837
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from Discosoma striata (coral)
  
 
===References===
 
===References===

Revision as of 13:23, 30 September 2018


Enigneered mutant of mRFP1 + RBS (compatible with all iGEM RFC assembly standards)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains improved version of the BBa_E1010 red flourescent protein. To make the BioBrick compatible to assembly standard RFC[25], two silent mutations were made to remove two AgeI sites: The AgeI site (accggt) at 573 bp was changed to accagt. The AgeI site (accggt) at 685 bp was changed to acgggt.

Part is originally derived from GenBank: AF506027.1

Source

GenBank: AF506027.1 https://www.ncbi.nlm.nih.gov/nuccore?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837 from Discosoma striata (coral)

References