Difference between revisions of "Part:BBa K2810001"

Revision as of 22:13, 26 September 2018

GUS reporter for plant expression

The GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry.

The GUS gene is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page].

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
-This has been used to generate the composite reporter constructs detailed in the 2018 Cardiff_Wales Parts page
+This has been used to generate the composite reporter constructs detailed in the [http://2018.igem.org/Team:Cardiff_Wales/Composite_Part 2018 Cardiff_Wales Composite Parts page].
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