Difference between revisions of "Part:BBa K2632001"

 
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<partinfo>BBa_K2381000 short</partinfo>
  
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pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is FAD which is normal in nearly all organisms.
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This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag
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<a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381001</a>.It's function is proved by parts                   
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<a href="https://parts.igem.org/Part:BBa_K2381017">BBa_K2381017</a>
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<br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.
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<h3>Reference</h3>
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<p>Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256</p>
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</html>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2381000 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2381000 parameters</partinfo>
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Revision as of 13:29, 15 September 2018

pMagFast

pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm2). Their light sensing molecule is FAD which is normal in nearly all organisms. This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag BBa_K2381001.It's function is proved by parts BBa_K2381017
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.

Reference

Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]