Generator

Part:BBa_K2381017

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-23)


light-activated rational split dCas9

the light-activated split dCas9, which fused the split dCas9 with light-induced dimerization protein, can be activated under the irritation of blue light(470nm)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3093


[edit]
Categories
Parameters
None