Difference between revisions of "Part:BBa K2810008"
 
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This part is taken from the 5' region of the SP3 gene, from the aphid <i>Myzus persicae </i>, made complementary, and expressed in <i> Nicotiana benthamiana </i> to test its effects on the aphid. The hope is that it will prevent formation of aphid bacteriocytes and thus prevent their symbiotic relationship with <i> Buchnera aphidicola </i>, killing the bacteria and the host.
 
This part is taken from the 5' region of the SP3 gene, from the aphid <i>Myzus persicae </i>, made complementary, and expressed in <i> Nicotiana benthamiana </i> to test its effects on the aphid. The hope is that it will prevent formation of aphid bacteriocytes and thus prevent their symbiotic relationship with <i> Buchnera aphidicola </i>, killing the bacteria and the host.
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SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid  bacteriocyte cells].
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This sequence is the complimentary sequence to the coding strand from a 5’ region of the Myzus persisae SP3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.
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SP3_5_3 is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
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It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
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This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in [https://parts.igem.org/Part:BBa_K2810007 BBa_K28100087].
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Latest revision as of 09:30, 3 September 2018


siRNA* from 5' region of Myzus persicae SP3 secreted protein

This part is taken from the 5' region of the SP3 gene, from the aphid Myzus persicae , made complementary, and expressed in Nicotiana benthamiana to test its effects on the aphid. The hope is that it will prevent formation of aphid bacteriocytes and thus prevent their symbiotic relationship with Buchnera aphidicola , killing the bacteria and the host.

SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid bacteriocyte cells].

This sequence is the complimentary sequence to the coding strand from a 5’ region of the Myzus persisae SP3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.

SP3_5_3 is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].

It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the complimentary sequence found in BBa_K28100087.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]