Difference between revisions of "Part:BBa K2810008"
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SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids bacteriocyte cells]. | SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids bacteriocyte cells]. | ||
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It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | ||
| − | This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in [https://parts.igem.org/Part: | + | This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in [https://parts.igem.org/Part:BBa_K2810007 BBa_K2810007]. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 10:42, 31 August 2018
siRNA* from 5' region of Myzus persicae SP3 secreted protein
SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids bacteriocyte cells].
This sequence is taken from the complement strand from a 5’ region of the Myzus persicae SP3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.
SP3_5_3 is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI or Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in BBa_K2810007.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]