Difference between revisions of "Part:BBa K2810004"
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It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | ||
| − | This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in | + | This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in [https://parts.igem.org/Part:BBa_K2810003 BBa_K2810003]. |
Latest revision as of 18:27, 24 August 2018
Complimentary sequence to Myzus persisae BCR3 secreted protein
BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid bacteriocyte cells].
This is the a complimentary sequence to the coding sequence of from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi. When expressed in an RNAi construct it is designed to form a hairpin with the BCR3_full sequence.
BCR3_full_comp is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].
It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in BBa_K2810003.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]