Difference between revisions of "Part:BBa K2810004"

Revision as of 18:26, 24 August 2018

Complimentary sequence to Myzus persisae BCR3 secreted protein

BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid bacteriocyte cells].

This is the a complimentary sequence to the coding sequence of from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi. When expressed in an RNAi construct it is designed to form a hairpin with the BCR3_full sequence.

BCR3_full_comp is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].

It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in [BBa_K2810003.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2810004 short</partinfo>
-BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids  bacteriocyte cells].
+BCR3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphid bacteriocyte cells].
-This is the a complimentary sequence to the coding sequence of from the Myzus persisae BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi. When expressed in an RNAi construct it is designed to form a hairpin with the [https://parts.igem.org/Part:BBa_K2810003 BCR3_full sequence].
+This is the a complimentary sequence to the coding sequence of from the <i>Myzus persisae</i> BCR3 gene that the Cardiff_Wales team is attempting to target using RNAi. When expressed in an RNAi construct it is designed to form a hairpin with the [https://parts.igem.org/Part:BBa_K2810003 BCR3_full sequence].
 BCR3_full_comp is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].