Difference between revisions of "Part:BBa K2810002"
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| + | The [https://academic.oup.com/treephys/article/32/1/83/1669058 Rice Tungro Bacilliform Virus (RTBV) promotor] has a vascular expression pattern in plants. This promotor has been added to the registry to facilitate expression of genes into plant vascular tissues. | ||
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| + | The RTBV promotor is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence. | ||
Revision as of 17:56, 24 August 2018
RTBV Vascular Specific Promotor
The Rice Tungro Bacilliform Virus (RTBV) promotor has a vascular expression pattern in plants. This promotor has been added to the registry to facilitate expression of genes into plant vascular tissues.
The RTBV promotor is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence.