Difference between revisions of "Part:BBa K2810001"
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<partinfo>BBa_K2810001 short</partinfo> | <partinfo>BBa_K2810001 short</partinfo> | ||
| − | The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry. | + | The [https://en.wikipedia.org/wiki/GUS_reporter_system GUS reporter gene system] is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry. |
The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | The GUS gene is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid. | ||
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This has been used to generate the composite reporter constructs detailed in the 2018 Cardiff_Wales Parts page | This has been used to generate the composite reporter constructs detailed in the 2018 Cardiff_Wales Parts page | ||
| + | ADD MORE ABOUT PARTS | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 17:32, 24 August 2018
GUS reporter for plant expression
The GUS reporter gene system is commonly used in plant molecular biology for visualization of expression patterns. This is the first addition of this important reporter gene to the iGEM registry.
The GUS gene is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.
This has been used to generate the composite reporter constructs detailed in the 2018 Cardiff_Wales Parts page
ADD MORE ABOUT PARTS
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]