Difference between revisions of "Part:BBa K2442399"
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*[https://parts.igem.org/Part:BBa_K2442402 K2442402] B0032 + split-T7 RNAP C-term | *[https://parts.igem.org/Part:BBa_K2442402 K2442402] B0032 + split-T7 RNAP C-term | ||
| − | *[https://parts.igem.org/Part:BBa_K2442409 | + | *[https://parts.igem.org/Part:BBa_K2442409 K2442409] [split T7 RNAP module 2] lacI regulated promoter + RBS + T7 rnap C-term + double terminator |
*[https://parts.igem.org/Part:BBa_K2442410 K2442410] Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2] | *[https://parts.igem.org/Part:BBa_K2442410 K2442410] Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2] | ||
Latest revision as of 03:43, 2 November 2017
T7 RNA Polymerase split C-term
This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.
This T7 RNA Polymerase split C-term coding sequence part did not exist, as it was directly PCR amplified with the B0032 ribosome binding site - this composite is K2442402.
Usage and Biology
This nonexistent part was used for assembly of larger composite split-T7 RNAP AND-gate constructs.
- K2442402 B0032 + split-T7 RNAP C-term
- K2442409 [split T7 RNAP module 2] lacI regulated promoter + RBS + T7 rnap C-term + double terminator
- K2442410 Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]