Part:BBa_K2442402

B0032 + T7 RNA Polymerase split C-term

This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-T7 RNAP AND-gate

Usage and Biology

This part was used for assembly of larger composite split-T7 RNAP AND-gate constructs.

• K2442409 [split T7 RNAP module 2] lacI regulated promoter + RBS + T7 rnap C-term + double terminator
• K2442410 Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]