Difference between revisions of "Part:BBa K2442399"
Line 10: Line 10:
  
  
 +
This T7 RNA Polymerase split C-term coding sequence part did not exist, as it was directly PCR amplified with the B0032 ribosome binding site - this composite is [https://parts.igem.org/Part:BBa_K2442402 K2442402].
  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
This nonexistent part was used for assembly of larger composite split-T7 RNAP AND-gate constructs.
 +
 +
*[https://parts.igem.org/Part:BBa_K2442402 K2442402] B0032 + split-T7 RNAP C-term
 +
*[https://parts.igem.org/Part:BBa_K2442409 K2442406] [split T7 RNAP module 2] lacI regulated promoter + RBS + T7 rnap C-term + double terminator
 +
*[https://parts.igem.org/Part:BBa_K2442410 K2442410] Full split-T7 RNAP AND-gate circuit [Split RNAP module 1 + Split RNAP module 2]
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 03:41, 2 November 2017


T7 RNA Polymerase split C-term


This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-T7 RNAP AND-gate


This T7 RNA Polymerase split C-term coding sequence part did not exist, as it was directly PCR amplified with the B0032 ribosome binding site - this composite is K2442402.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]