Difference between revisions of "Part:BBa K2442403"

Latest revision as of 03:28, 2 November 2017

GFP split N-term (GFP2) + B0015 double terminator

This subproject is discussed in full detail on our [http://2017.igem.org/Team:Glasgow/ANDGate AND-Gate] wiki page.

A schematic representation of a final split-GFP AND-gate

This is a composite of the GFP N-term split subunit (GFP1) K1789003 and the well characterised double-terminator construct B0015.

This N-term GFP part was found to contain an extra A nucleotide immediately preceding the start-codon ATG. This erroneous nucleotide is present in the parts-registry sequencing for K1789003, but is not reported as an error. Unfortunately, now both split-GFP modules might be affected by improperly spaced ribosome binding sites. Despite this we proceeded to the next assembly stage.

A Biobrick prefix error in GFP N-term part.</b> Alignment of a sequencing read of the split GFP N-terminal to a reference sequence plasmid map. The base highlighted in red indicate an insertion has occurred at those positions relative to the reference sequence. The single-A insertion may correspond to the use a taq-polymerase during the original amplification of this coding sequence. Alignments performed in ApE.

Usage and Biology

this composite was used for assembly of larger composite split-GFP AND-gate constructs.

• K2442405 [split GFP module 1] tetR repressible promoter + GFP N-term + RBS + double terminator
• K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]

Sequence and Features