Difference between revisions of "Part:BBa K2213009"

Revision as of 03:03, 2 November 2017

LowPromoter_PduD(1-20)_mCherry_cgPPK2
The PduD(1-20) localisation tag, mCherry and PPK (from C. glutamicum) under control of the low strength Anderson promoter (https://parts.igem.org/Part:BBa_J23105). PPK is tagged with His6 (see figure 1).

Figure 1: Schematic of LowPromoter_PduD(1-20)_mCherry_cgPPK2

The Manchester iGEM team has combined this part with BBa_K2213002, which contains EutLK under the control of an araBad promoter, to make the composite part, BBa_K2213013. Coexpression of BBa_K2213013 and the other composite part, BBa_K2213012 (see: https://parts.igem.org/Part:BBa_K2213012) a showed that cgPPk2 was localised in the Eut microcompartment, and served as a proof of concept for the Manchester team's project. Characterisation of BBa_K2213013 can be found at: https://parts.igem.org/Part:BBa_K2213013 and characterisation of BBa_K2213002 can be found at: https://parts.igem.org/Part:BBa_K2213002.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 8
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 908
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 146
Illegal SapI.rc site found at 1587

@@ Line 8: / Line 8: @@
 <b>Figure 1:</b> Schematic of LowPromoter_PduD(1-20)_mCherry_cgPPK2<br>
 <br>
-The Manchester iGEM team has combined this part with BBa_K2213002, which contains EutLK under the control of an araBad promoter, to make the composite part, BBa_K2213013. Subsequent experiments with BBa_K2213013 showed that cgPPk2 was localised in the Eut microcompartment, and served as a proof of concept for the Manchester team's project. Characterisation of BBa_K2213013 can be found at: https://parts.igem.org/Part:BBa_K2213013 and characterisation of BBa_K2213002 can be found at: https://parts.igem.org/Part:BBa_K2213002.
+The Manchester iGEM team has combined this part with BBa_K2213002, which contains EutLK under the control of an araBad promoter, to make the composite part, BBa_K2213013. Coexpression of BBa_K2213013 and the other composite part, BBa_K2213012 (see: https://parts.igem.org/Part:BBa_K2213012) a showed that cgPPk2 was localised in the Eut microcompartment, and served as a proof of concept for the Manchester team's project. Characterisation of BBa_K2213013 can be found at: https://parts.igem.org/Part:BBa_K2213013 and characterisation of BBa_K2213002 can be found at: https://parts.igem.org/Part:BBa_K2213002.
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