Difference between revisions of "Part:BBa I739001"

(→‎Purpose)
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===Part Structure===
 
===Part Structure===
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged TetR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0040 BBa_C0040]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of tetR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
+
<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged TetR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0040 BBa_C0040]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23105 BBa_J23105] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of tetR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
  
 
===Mode of Action===
 
===Mode of Action===
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===Purpose===
 
===Purpose===
−
<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739102 BBa_I739102] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739106 BBa_I739106] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739010 BBa_I739010] respectively. A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p>
+
<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739102 BBa_I739102] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739106 BBa_I739106] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739010 BBa_I739010] respectively. A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p>
  
 
===Testing===
 
===Testing===

Revision as of 19:44, 26 October 2007

Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)

Part Structure

The Biobrick encodes LVA-tagged TetR (BBa_C0040) under control of the constitutive promoter BBa_J23105 followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.

Mode of Action

TetR binds to the pTet regulator (BBa_R0040) and represses transcription. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, TetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 1. The constitutively synthesized TetR interacts with the double promoters BBa_I739102 and BBa_I739106 which are parts of composites BBa_I739015 and BBa_I739010 respectively. A similar (but not used) construct in this context is BBa_Q04400.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]