Difference between revisions of "Part:BBa K2455002:Design"
(→Design Notes) |
(→Design Notes) |
||
| Line 13: | Line 13: | ||
Reverse primer: CACGCGAUGA-TTTATACAGCTCATCCATACCCAGGG | Reverse primer: CACGCGAUGA-TTTATACAGCTCATCCATACCCAGGG | ||
| − | Subsequently the PCR product could be gel-extracted and ligated into the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette] biobrick. | + | Subsequently the PCR product could be gel-extracted and ligated into the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette]] biobrick. |
Finally for the creation of a biobrick version the following set of primers were used: | Finally for the creation of a biobrick version the following set of primers were used: | ||
Revision as of 00:20, 2 November 2017
CPP-SYFP2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The first step in the insertion was amplification of SYFP2 using primers matching the USER Cassette of the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette]. To do this we used the following primers:
Forward primer: CGTGCGAUCA-ATGGTTAGCAAGGGCGAAG
Reverse primer: CACGCGAUGA-TTTATACAGCTCATCCATACCCAGGG
Subsequently the PCR product could be gel-extracted and ligated into the Cell-Penetrating USER Cassette biobrick.
Finally for the creation of a biobrick version the following set of primers were used:
Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC
Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG
Source
Needed