Difference between revisions of "Part:BBa K2455002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Needed
+
The first step in the insertion was amplification of [[Part:BBa_K864100|SYFP2]] using primers matching the USER Cassette of the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette]. To do this we used the following primers:
  
 +
Forward primer: CGTGCGAUCA-ATGGTTAGCAAGGGCGAAG
  
 +
Reverse primer: CACGCGAUGA-TTTATACAGCTCATCCATACCCAGGG
 +
 +
Subsequently the PCR product could be gel-extracted and ligated into the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette] biobrick.
 +
 +
Finally for the creation of a biobrick version the following set of primers were used:
 +
 +
Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC
 +
 +
Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG
  
 
===Source===
 
===Source===

Revision as of 00:20, 2 November 2017


CPP-SYFP2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The first step in the insertion was amplification of SYFP2 using primers matching the USER Cassette of the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette]. To do this we used the following primers:

Forward primer: CGTGCGAUCA-ATGGTTAGCAAGGGCGAAG

Reverse primer: CACGCGAUGA-TTTATACAGCTCATCCATACCCAGGG

Subsequently the PCR product could be gel-extracted and ligated into the [[Part:BBa_K2455003|Cell-Penetrating USER Cassette] biobrick.

Finally for the creation of a biobrick version the following set of primers were used:

Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC

Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG

Source

Needed

References