Difference between revisions of "Part:BBa K2446031"

 
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<partinfo>BBa_K2446031 short</partinfo>
 
<partinfo>BBa_K2446031 short</partinfo>
  
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waiting
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This part is a mammalian synthetic promoter (SynPro) based on SV40 promoter (<partinfo>BBa_K2446052</partinfo>) and TF binding sites. We inset the TF binding sites before the promoter. minSV40 promoter is a minimized promoter from human. In this way, we can use our mammalian synthetic transcription factors (SynTFs) which is a fusing protein TF-DBD_(G4S) linker_NLS_KRAB. The TF-KRAB can bind to the TF binding sites specificallyand the KRAB domain can active the expression of minSV40 promoter [[#References|[1]]]. It can use as a "NAND" gate regulated by SynTF-KRAB.
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[[file:T--Fudan--design.png|500px|middle|'''Figure 1''': The design of SynTF-SynPros.]]
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<div>
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Figure 1: The design of SynTF-SynPros.
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</div>
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But for the limitation of time, we haven’t complete the test of this SynPros. Another group of SynTF-KRAB&SynPros(Sv40) works well. They are all in the table below.
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<table border="2"  width="800px" >
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<tr>
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<th>SynTFs </th>
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<th>SynPros</th>
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</tr>
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<tr>
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<td>Gal4-KRAB(TF-KRAB-1)  <partinfo>BBa_K2446037</partinfo></td>
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<td>Sv40-UAS(Sv40-UAS) <partinfo>BBa_K2446036</partinfo></td>
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</tr>
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<tr>
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<td>ZF_PIP_KRAB(TF-KRAB-2) <partinfo>BBa_K2446045</partinfo></td>
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<td>SV40_2/4/8_PIP <partinfo>BBa_K2446033</partinfo>/<partinfo>BBa_K2446034</partinfo>/<partinfo>BBa_K2446035</partinfo></td>
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</tr>
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<tr>
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<td>ZF_21-16KRAB(TF-KRAB-3) <partinfo>BBa_K2446039 </partinfo></td>
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<td>SV40_8_ZF_21-16 <partinfo>BBa_K2446030</partinfo></td>
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</tr>
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<tr>
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<td>ZF_42-10_KRAB(TF-KRAB-4) <partinfo>BBa_K2446040</partinfo></td>
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<td>SV40_8_ZF_42-10 <partinfo>BBa_K2446025</partinfo></td>
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</tr>
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<tr>
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<td>ZF_43-8_KRAB(TF-KRAB-5) <partinfo>BBa_K2446041</partinfo></td>
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<td>SV40_2/4/8_ZF_43-8 <partinfo>BBa_K2446026</partinfo>/<partinfo>BBa_K2446027</partinfo>/<partinfo>BBa_K2446028</partinfo></td>
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</tr>
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<tr>
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<td>ZF_54-8_KRAB(TF-KRAB-6) <partinfo>BBa_K2446042</partinfo></td>
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<td>SV40_8_ZF_54-8 <partinfo>BBa_K2446029</partinfo></td>
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</tr>
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<tr>
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<td>ZFHD1_KRAB(TF-KRAB-7) <partinfo>BBa_K2446043</partinfo></td>
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<td>SV40_4_ZFHD1 <partinfo>BBa_K2446032</partinfo></td>
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</tr>
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</table>
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<partinfo>BBa_K2446031 parameters</partinfo>
 
<partinfo>BBa_K2446031 parameters</partinfo>
 
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===References===
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[1] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).

Revision as of 20:56, 1 November 2017


SV40_8_ZF54-8/43-8 promoter

This part is a mammalian synthetic promoter (SynPro) based on SV40 promoter (BBa_K2446052) and TF binding sites. We inset the TF binding sites before the promoter. minSV40 promoter is a minimized promoter from human. In this way, we can use our mammalian synthetic transcription factors (SynTFs) which is a fusing protein TF-DBD_(G4S) linker_NLS_KRAB. The TF-KRAB can bind to the TF binding sites specificallyand the KRAB domain can active the expression of minSV40 promoter [1]. It can use as a "NAND" gate regulated by SynTF-KRAB. Figure 1: The design of SynTF-SynPros.

Figure 1: The design of SynTF-SynPros.

But for the limitation of time, we haven’t complete the test of this SynPros. Another group of SynTF-KRAB&SynPros(Sv40) works well. They are all in the table below.

SynTFs SynPros
Gal4-KRAB(TF-KRAB-1) BBa_K2446037 Sv40-UAS(Sv40-UAS) BBa_K2446036
ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035
ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 SV40_8_ZF_21-16 BBa_K2446030
ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 SV40_8_ZF_42-10 BBa_K2446025
ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028
ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 SV40_8_ZF_54-8 BBa_K2446029
ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 SV40_4_ZFHD1 BBa_K2446032


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).