Difference between revisions of "Part:BBa K2259040"
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<partinfo>BBa_K2259040 short</partinfo> | <partinfo>BBa_K2259040 short</partinfo> | ||
− | + | This is a full composite part of the activating RNA Trigger 2 [[part:BBa_K2259017]]. It has a constant expression by [[part:BBa_J23101]] promoter and a double terminator at the end. | |
+ | This part is used together with [[part:BBa_K2259035]] to build a 3 or 4 plasmid SynORI selection gene circuit. | ||
+ | Trigger 2 part activates the translation of Toehold 2 [[part:BBa_K2259015]] locked gene. | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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=Introduction= | =Introduction= | ||
− | == | + | ==The overview of 4 plasmid system== |
− | + | ||
− | [[Image: | + | [[Image:4plasmid.png|center|500px|thumb|<b>Figure 1. </b> The principle scheme of SynORI 4 plasmid selection system]] |
− | + | ||
+ | ==The overview of 5 plasmid system== | ||
− | <b> | + | [[Image:5plasmid.png|center|500px|thumb|<b>Figure 1. </b> The principle scheme of SynORI 4 plasmid selection system]] |
− | + | == Results == | |
− | + | [[Image:5plasmidresult.jpg|center|500px|thumb|<b>Figure 1. </b> The results of 5 plasmid co-transformation using SynORI 5 plasmid selection system]] | |
− | + | =About SynORI= | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
[[Image:Aboutsynoritry1.png|600px|center|]] | [[Image:Aboutsynoritry1.png|600px|center|]] | ||
SynORI is a framework for multi-plasmid systems created by ''Vilnius-Lithuania 2017'' which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]! | SynORI is a framework for multi-plasmid systems created by ''Vilnius-Lithuania 2017'' which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]! | ||
− | == | + | ==Toehold riboregulators in SynORI== |
− | RNA | + | Toehold switches together with their corresponding RNA triggers and split antibiotic genes completes the dynamic SynORI selection system. The switches lock the translation of downstream split antibiotic genes and form an AND type gate genetic circuit which functions to stably maintain multiple plasmids in the SynORI collection. |
− | The | + | |
− | + | ||
− | + | ||
+ | SynORI selection gene circuits for multi-plasmid systems: | ||
− | + | • 2 plasmids | |
− | + | Consisting of: : Two split antibiotic genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]) | |
− | + | • 3 plasmids | |
− | + | ||
− | [[ | + | Consisting of: |
+ | One Toehold ([[part:BBa_K2259014]] or [[part:BBa_K2259015]]), | ||
+ | one Trigger RNA ([[part:BBa_K2259016]] or [[part:BBa_K2259017]]) and | ||
+ | split neomycin antibiotic resistance genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]). | ||
− | + | • 4 plasmids | |
− | + | ||
+ | Consisting of: Two Toeholds ([[part:BBa_K2259014]] and [[part:BBa_K2259015]]), two Trigger RNAs ([[part:BBa_K2259016]] and [[part:BBa_K2259017]]) and split neomycin antibiotic resistance genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]). | ||
+ | |||
+ | • 5 plasmids | ||
+ | |||
+ | Consisting of: Modified phage control system [[part:BBa_K2259044]], two Toeholds ([[part:BBa_K2259014]] and [[part:BBa_K2259015]]), two repressed Trigger RNAs ([[part:BBa_K2259042]] and [[part:BBa_K2259043]]) and split neomycin antibiotic resistance genes ([[part:BBa_K2259018]] and [[part:BBa_K2259019]]). | ||
+ | |||
+ | ===Two groups of Toeholds=== | ||
+ | SynORI collection introduces two Toehold sequences termed Toehold 1 and Toehold 2 which only interact with its corresponding Trigger RNA, termed Trigger 1 and Trigger 2 and display no cross interaction. | ||
− | |||
− | |||
− | |||
− | |||
==References== | ==References== | ||
− | + | Toehold Switches: De-Novo-Designed Regulators of Gene Expression | |
+ | |||
+ | Green, Alexander A. et al. | ||
+ | Cell, Volume 159, Issue 4, 925 - 939 |
Revision as of 23:09, 31 October 2017
Trigger 2 for toehold activation (SynORI framework)
This is a full composite part of the activating RNA Trigger 2 part:BBa_K2259017. It has a constant expression by part:BBa_J23101 promoter and a double terminator at the end.
This part is used together with part:BBa_K2259035 to build a 3 or 4 plasmid SynORI selection gene circuit.
Trigger 2 part activates the translation of Toehold 2 part:BBa_K2259015 locked gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Contents
Introduction
The overview of 4 plasmid system
The overview of 5 plasmid system
Results
About SynORI
SynORI is a framework for multi-plasmid systems created by Vilnius-Lithuania 2017 which enables quick and easy workflow with multiple plasmids, while also allowing to freely pick and modulate copy number for every unique plasmid group! Read more about [http://2017.igem.org/Team:Vilnius-Lithuania SynORI here]!
Toehold riboregulators in SynORI
Toehold switches together with their corresponding RNA triggers and split antibiotic genes completes the dynamic SynORI selection system. The switches lock the translation of downstream split antibiotic genes and form an AND type gate genetic circuit which functions to stably maintain multiple plasmids in the SynORI collection.
SynORI selection gene circuits for multi-plasmid systems:
• 2 plasmids
Consisting of: : Two split antibiotic genes (part:BBa_K2259018 and part:BBa_K2259019)
• 3 plasmids
Consisting of: One Toehold (part:BBa_K2259014 or part:BBa_K2259015), one Trigger RNA (part:BBa_K2259016 or part:BBa_K2259017) and split neomycin antibiotic resistance genes (part:BBa_K2259018 and part:BBa_K2259019).
• 4 plasmids
Consisting of: Two Toeholds (part:BBa_K2259014 and part:BBa_K2259015), two Trigger RNAs (part:BBa_K2259016 and part:BBa_K2259017) and split neomycin antibiotic resistance genes (part:BBa_K2259018 and part:BBa_K2259019).
• 5 plasmids
Consisting of: Modified phage control system part:BBa_K2259044, two Toeholds (part:BBa_K2259014 and part:BBa_K2259015), two repressed Trigger RNAs (part:BBa_K2259042 and part:BBa_K2259043) and split neomycin antibiotic resistance genes (part:BBa_K2259018 and part:BBa_K2259019).
Two groups of Toeholds
SynORI collection introduces two Toehold sequences termed Toehold 1 and Toehold 2 which only interact with its corresponding Trigger RNA, termed Trigger 1 and Trigger 2 and display no cross interaction.
References
Toehold Switches: De-Novo-Designed Regulators of Gene Expression
Green, Alexander A. et al. Cell, Volume 159, Issue 4, 925 - 939