Difference between revisions of "Part:BBa K2276010:Design"
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Borujeni A E, Salis H M. Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism[J]. Journal of the American Chemical Society, 2016, 138(22):7016. | Borujeni A E, Salis H M. Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism[J]. Journal of the American Chemical Society, 2016, 138(22):7016. | ||
| − | Part:BBa_I13521 | + | https://parts.igem.org/Part:BBa_I13521:Design |
Revision as of 07:51, 31 October 2017
a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 635
Illegal AgeI site found at 747 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We want to engineer a strain which can produce melatonin at daily rhythm.We employ repressilator system thus allowing the E.coli coding enzyme periodically. However, the cycle time is not 24h.We built a model to analyse the issue.According to the medeling result, a stronger RBS was needed.We turned to the bioinformatic methods to design a RBS predicted to show hign translation rate.This is the website on which we performed the design: https://salislab.net/software/doForwardRBS. We designed three, and BBa_K2276010 is one of them.
Source
Part:BBa_I13521
References
Borujeni A E, Salis H M. Translation Initiation is Controlled by RNA Folding Kinetics via a Ribosome Drafting Mechanism[J]. Journal of the American Chemical Society, 2016, 138(22):7016.