Reporter

Part:BBa_I13521:Design

Designed by: jkm   Group: iGEM04_MIT   (2005-06-22)

Ptet mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

Fwd: GCTTCTAGAGATACATGAACATGCAATACtccctatcagtgatagagattgacatcc

Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg

Source

References

The improvements submitted by iGEM17_SCU-China

https://parts.igem.org/Part:BBa_K2276007 https://parts.igem.org/Part:BBa_K2276010