Reporter
Part:BBa_I13521:Design
Designed by: jkm Group: iGEM04_MIT (2005-06-22)
Ptet mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 635
Illegal AgeI site found at 747 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.
Fwd: GCTTCTAGAGATACATGAACATGCAATACtccctatcagtgatagagattgacatcc
Rv: CTGCAGCGGCCGCTACTAGTAGAGAGCGTTCtataaacgcagaaaggcccacccg
Source
References
The improvements submitted by iGEM17_SCU-China
https://parts.igem.org/Part:BBa_K2276007 https://parts.igem.org/Part:BBa_K2276010