Difference between revisions of "Part:BBa K2446039"

 
Line 3: Line 3:
 
<partinfo>BBa_K2446039 short</partinfo>
 
<partinfo>BBa_K2446039 short</partinfo>
  
−
waiting
+
<div>
 +
This part is one of our mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. [[#References|[1]]]. ZF_21-16_KRAB (or TF-KRAB-3 in our project) containing three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [[#References|[2]]].And a (G4S) linker was added between DBD and NLS for providing region flexibility [[#References|[3]]]. ZF_21-16-DBD enable binding to specific DNA sequences(as figure 1 show), so that we can use ZF_21-16-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter SV40-8_ZF_21-16 ( <partinfo>BBa_K2446030</partinfo>).
 +
</div>
 +
[[file:T--Fudan--design%26ZF.png|800px|middle|'''Figure 1''':(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used]]
 +
<div>
 +
<B> Figure 1:(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used</B>
 +
</div>
 +
The information of other SynTF-SynPros is showed in the table below.
 +
<table border="2"  width="800px" >
 +
<tr>
 +
<th>SynTFs </th>
 +
<th>SynPros</th>
 +
</tr>
 +
<tr>
 +
<td>Gal4-KRAB(TF-KRAB-1)  <partinfo>BBa_K2446037</partinfo></td>
 +
<td>Sv40-UAS(Sv40-UAS) <partinfo>BBa_K2446036</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_PIP_KRAB(TF-KRAB-2) <partinfo>BBa_K2446045</partinfo></td>
 +
<td>SV40_2/4/8_PIP <partinfo>BBa_K2446033</partinfo>/<partinfo>BBa_K2446034</partinfo>/<partinfo>BBa_K2446035</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_21-16KRAB(TF-KRAB-3) <partinfo>BBa_K2446039 </partinfo></td>
 +
<td>SV40_8_ZF_21-16 <partinfo>BBa_K2446030</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_42-10_KRAB(TF-KRAB-4) <partinfo>BBa_K2446040</partinfo></td>
 +
<td>SV40_8_ZF_42-10 <partinfo>BBa_K2446025</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_43-8_KRAB(TF-KRAB-5) <partinfo>BBa_K2446041</partinfo></td>
 +
<td>SV40_2/4/8_ZF_43-8 <partinfo>BBa_K2446026</partinfo>/<partinfo>BBa_K2446027</partinfo>/<partinfo>BBa_K2446028</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZF_54-8_KRAB(TF-KRAB-6) <partinfo>BBa_K2446042</partinfo></td>
 +
<td>SV40_8_ZF_54-8 <partinfo>BBa_K2446029</partinfo></td>
 +
</tr>
 +
<tr>
 +
<td>ZFHD1_KRAB(TF-KRAB-7) <partinfo>BBa_K2446043</partinfo></td>
 +
<td>SV40_4_ZFHD1 <partinfo>BBa_K2446032</partinfo></td>
 +
</tr>
 +
</table>
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:09, 31 October 2017


ZF_21-16KRAB

This part is one of our mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. [1]. ZF_21-16_KRAB (or TF-KRAB-3 in our project) containing three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain, nuclear location sequence and KRAB transcription regulating domain [2].And a (G4S) linker was added between DBD and NLS for providing region flexibility [3]. ZF_21-16-DBD enable binding to specific DNA sequences(as figure 1 show), so that we can use ZF_21-16-KRAB as a specific transcription factors to repress the expression of our mammalian synthetic promoter SV40-8_ZF_21-16 ( BBa_K2446030).

Figure 1:(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used

Figure 1:(A)Design of SynTF-SynPros. (B) The specific binding sites sequences of Zinc finger we used

The information of other SynTF-SynPros is showed in the table below.

SynTFs SynPros
Gal4-KRAB(TF-KRAB-1) BBa_K2446037 Sv40-UAS(Sv40-UAS) BBa_K2446036
ZF_PIP_KRAB(TF-KRAB-2) BBa_K2446045 SV40_2/4/8_PIP BBa_K2446033/BBa_K2446034/BBa_K2446035
ZF_21-16KRAB(TF-KRAB-3) BBa_K2446039 SV40_8_ZF_21-16 BBa_K2446030
ZF_42-10_KRAB(TF-KRAB-4) BBa_K2446040 SV40_8_ZF_42-10 BBa_K2446025
ZF_43-8_KRAB(TF-KRAB-5) BBa_K2446041 SV40_2/4/8_ZF_43-8 BBa_K2446026/BBa_K2446027/BBa_K2446028
ZF_54-8_KRAB(TF-KRAB-6) BBa_K2446042 SV40_8_ZF_54-8 BBa_K2446029
ZFHD1_KRAB(TF-KRAB-7) BBa_K2446043 SV40_4_ZFHD1 BBa_K2446032


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]