Difference between revisions of "Part:BBa K2404003"
 
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This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
 
This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
 
   
 
   
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This part is has been cloned into the pSB1C3 plasmid using BsmBI.
+
This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI.
  
  

Latest revision as of 21:20, 30 October 2017


PR2 shortened - a salicylic acid-induced promoter

The PR2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10061089.x/full respond to salicylic acid].

This promotor is taken from sequence -642 > -145 upstream of the PR2 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the P10500 plasmid using BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 238
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 514