Difference between revisions of "Part:BBa K2382013"

(→‎References)
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
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This is a fragment of <partinfo>BBa_K2382010</partinfo>. We designed a restriction site, BamHI, before EAAAK rigid
 
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linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as
 
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indicator.
 
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This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have
 
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the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein
 
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with this part may have red color and the ability to be purified easily.
 
  
 
===Characterization of the EAAAK+ RFP+Histag===
 
===Characterization of the EAAAK+ RFP+Histag===

Revision as of 05:58, 30 October 2017

EAAAK+ RFP+Histag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

Characterization of the EAAAK+ RFP+Histag

References

BioBrick Part BBa_E1010