Difference between revisions of "Part:pSB4C1"
 
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__NOTOC__
 
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<partinfo>BBa_I739202 short</partinfo>
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<partinfo>pSB4C1 short</partinfo>
  
 
Plasmid pCK01BB1 (Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.<br>
 
Plasmid pCK01BB1 (Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.<br>
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In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. Since the original plasmid also contains a SpeI restriction site in the origin of replication, the ACT codon at this position was changed to ATT by site directed mutagenesis. The term "BB1" in pCK01BB1 refers to "BioBrick version 1".
 
In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. Since the original plasmid also contains a SpeI restriction site in the origin of replication, the ACT codon at this position was changed to ATT by site directed mutagenesis. The term "BB1" in pCK01BB1 refers to "BioBrick version 1".
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===Purpose===
 
===Purpose===
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<p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''], [https://parts.igem.org/wiki/index.php/Part:BBa_E0430  ''Part 5new''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739008 ''Part 8''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739009 ''Part 9''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015], [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739017 BBa_I739017]. More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here].
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<p>This plasmid was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''], [https://parts.igem.org/wiki/index.php/Part:BBa_E0430  ''Part 5new''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739008 ''Part 8''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739009 ''Part 9''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015], [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739017 BBa_I739017]. More information on the plasmid and the used assembly strategy can be found [http://2007.igem.org?title=ETHZ/Biology/Lab here].
 
</p><br>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_I739202 SequenceAndFeatures</partinfo>
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<partinfo>pSB4C1 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_I739202 parameters</partinfo>
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<partinfo>pSB4C1 parameters</partinfo>
 
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Latest revision as of 08:40, 25 October 2007

pCK01 converted into BioBrick vector (formerly BBa_I739202)

Plasmid pCK01BB1 (Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.

Fig. 1: Map of the pCK01BB1 plasmid
Fig. 2: Map of the original pCK01 plasmid

In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. Since the original plasmid also contains a SpeI restriction site in the origin of replication, the ACT codon at this position was changed to ATT by site directed mutagenesis. The term "BB1" in pCK01BB1 refers to "BioBrick version 1".

Purpose

This plasmid was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 4, Part 5new, Part 8, Part 9 and the composites BBa_I739015, BBa_I739016 and BBa_I739017. More information on the plasmid and the used assembly strategy can be found [http://2007.igem.org?title=ETHZ/Biology/Lab here].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3134
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3134
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3134
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3149
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 3128
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.