Difference between revisions of "Part:pSB4C1"
Stefan Luzi (Talk | contribs) |
Stefan Luzi (Talk | contribs) |
||
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
− | <partinfo> | + | <partinfo>BBa_I739202 short</partinfo> |
Plasmid pCK01BB1 (Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.<br> | Plasmid pCK01BB1 (Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.<br> | ||
Line 8: | Line 8: | ||
===Purpose=== | ===Purpose=== | ||
− | <p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''], [https://parts.igem.org/wiki/index.php/Part:BBa_E0430 ''Part 5new''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739008 ''Part 8''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739009 ''Part 9''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015], [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739017 BBa_I739017].<br><br>More information on the plasmid and the assembly strategy | + | <p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''], [https://parts.igem.org/wiki/index.php/Part:BBa_E0430 ''Part 5new''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739008 ''Part 8''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739009 ''Part 9''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015], [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739017 BBa_I739017].<br><br>More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here]. |
− | </p | + | </p><br> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 16: | Line 16: | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_I739202 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_I739202 parameters</partinfo> |
<!-- --> | <!-- --> |
Revision as of 23:30, 24 October 2007
No part name specified with partinfo tag.
Plasmid pCK01BB1 (Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.
In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced in this position. Since the original plasmid also contains a SpeI restriction site in the origin of replication, the ACT codon at this position was changed to ATT by site directed mutagenesis. The term "BB1" in pCK01BB1 refers to "BioBrick version 1".
Purpose
This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 4, Part 5new, Part 8, Part 9 and the composites BBa_I739015, BBa_I739016 and BBa_I739017.
More information on the plasmid and the used assembly strategy can be found [http://parts.mit.edu/igem07/index.php?title=ETHZ/Biology/Lab here].
Sequence and Features No part name specified with partinfo tag.