Difference between revisions of "Part:pSB4C1"
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[[Image:MapI739201.jpg|left|thumb|'''Fig. 1:''' Map of the pCK01BB1 plasmid|250px]][[Image:Mappcko1.jpg|right|thumb|'''Fig. 2:''' Map of the original pCK01 plasmid|250px]]
 
[[Image:MapI739201.jpg|left|thumb|'''Fig. 1:''' Map of the pCK01BB1 plasmid|250px]][[Image:Mappcko1.jpg|right|thumb|'''Fig. 2:''' Map of the original pCK01 plasmid|250px]]
  
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In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced at this position. Since the original plasmid also contains a SpeI restriction in the origin of replication, the ACT codon in this site was changed to ATT by site directed mutagenesis. The term "BB1" refers to "BioBrick version 1".
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In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced at this position. Since the original plasmid also contains a SpeI restriction in the origin of replication, the ACT codon in this site was changed to ATT by site directed mutagenesis. The term "BB1" in the name pCK01BB1 refers to "BioBrick version 1".
  
 
===Purpose===
 
===Purpose===
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<p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains... </p>
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<p>This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''], [https://parts.igem.org/wiki/index.php/Part:BBa_E0430 ''Part 5new''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739008 ''Part 8''], [https://parts.igem.org/wiki/index.php/Part:BBa_I739009 ''Part 9''] and the composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739015 BBa_I739015], [https://parts.igem.org/wiki/index.php/Part:BBa_I739016 BBa_I739016] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739017 BBa_I739017].</</</p>
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=== References ===
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<p>
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[1]  Fernández S, de Lorenzo V, Pérez-Martín J <i>"Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains"</i>, Mol Microbiol. 16(2):205-13, 2000</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:19, 24 October 2007

pBR322BB2

Plasmid pCK01BB1(Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.

Fig. 1: Map of the pCK01BB1 plasmid
Fig. 2: Map of the original pCK01 plasmid

In order to make this plasmid compatible with the BioBrick Standard assembly process, the original pCK01 was digested at its AgeI and AseI restriction sites and an appropriate multiple cloning site (MCS) was introduced at this position. Since the original plasmid also contains a SpeI restriction in the origin of replication, the ACT codon in this site was changed to ATT by site directed mutagenesis. The term "BB1" in the name pCK01BB1 refers to "BioBrick version 1".

Purpose

This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and is used for the following BioBricks: Part 4, Part 5new, Part 8, Part 9 and the composites BBa_I739015, BBa_I739016 and BBa_I739017.</</

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
    Illegal NotI site found at 3612
    Illegal NotI site found at 3645
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3638
    Illegal SpeI site found at 3621
    Illegal PstI site found at 3607
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3433
    Illegal SapI site found at 2350